|Application:||RNA-seq analysis, BS-seq analysis|
|Number of samples:||8|
|Release date:||Jul 11 2017|
|Last update date:||Jan 12 2018|
|Dataset link||The single-base resolution DNA methylation profiles for four developmental stages of Tribolium castaneum|
Genomic DNA was extracted from a pool of 50 individuals for the four life stages of Tribolium castaneum: 3d embryo, late larva( ~20d larva ), 3d pupa and 3d adult, respectively, using the DNeasy Blood & Tissue Kit. The extracted DNA was then bisulfite converted and Illumina sequenced to obtain direct evidence for the presence of methylation in Tribolium castaneum. To reveal the relationship between the DNA methylation and gene expression, Total RNA was extracted from each sample by using the Invitrogen TRIzol® Reagent. RNA-seq technology was used to generate the gene expression files for the four sages fo Tribolium castaneum.
Citations per year
DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes