Dataset features

Specifications


Application: RNA-seq analysis, BS-seq analysis
Number of samples: 8
Release date: Jul 11 2017
Last update date: Jan 12 2018
Access: Public
Diseases: Leukemia, T-Cell
Dataset link The single-base resolution DNA methylation profiles for four developmental stages of Tribolium castaneum

Experimental Protocol


Genomic DNA was extracted from a pool of 50 individuals for the four life stages of Tribolium castaneum: 3d embryo, late larva( ~20d larva ), 3d pupa and 3d adult, respectively, using the DNeasy Blood & Tissue Kit. The extracted DNA was then bisulfite converted and Illumina sequenced to obtain direct evidence for the presence of methylation in Tribolium castaneum. To reveal the relationship between the DNA methylation and gene expression, Total RNA was extracted from each sample by using the Invitrogen TRIzolĀ® Reagent. RNA-seq technology was used to generate the gene expression files for the four sages fo Tribolium castaneum.

Repositories


GEO

GSE84253

ENA

SRP078251

BioProject

PRJNA328479

Download


Contact


Bin Li

Dataset Statistics

info

Citations per year

Dataset publication