Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 9
Release date: Feb 27 2018
Last update date: Nov 30 2018
Access: Public
Computational protocol: HISAT2, TopHat, GEO
Dataset link Direct reprogramming of spiral ganglion non-neuronal cells into neurons: Towards ameliorating sensorineural hearing loss by gene therapy

Experimental Protocol


P1 Tau-EGFP cochleae were dissected and isolated SGs were sorted by EGFP fluorescence with flow cytometer. EGFP positive fraction contained endogenous PANs, and EGFP negative fraction containing SGNNCs. SGNNCs were cultured and transfected with transcription factors or control vector - induced neurons (iN) or vector control (VC) respectively. PANs, iNs, and VC cells were collected and RNA extracted for transcriptome analysis.

Repositories


GEO

GSE107461

ENA

SRP125778

BioProject

PRJNA420108

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Contact


Yutaka Amemiya

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