Computational protocol: Human Pancreatic β Cell lncRNAs Control Cell-Specific Regulatory Networks

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Protocol publication

[…] RNA-seq data have been described previously (). The samples were aligned to the hg19 genome using STAR aligner version 2.3.0 as described in the , quantification was carried out with HTseq-Count 0.6.1, and differential expression analysis of lncRNA genes was done using DEseq2 1.10 () using an adjusted p value threshold of 0.05. […]

Pipeline specifications

Software tools STAR, HTSeq, DESeq2
Application RNA-seq analysis
Organisms Homo sapiens
Diseases Diabetes Mellitus, Glucose Intolerance
Chemicals Glucose