Computational protocol: Inhibition of β-catenin signaling respecifies anterior-like endothelium into beating human cardiomyocytes

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Protocol publication

[…] Samples were submitted to University of Washington High-Throughput Genomic Sequencing Center for isolation and analysis. RNA-seq was performed on poly-A-enriched samples using Illumina TruSeq. Differentially expressed genes were classified according to gene ontology using the NIAID Database for Annotation, Visualization and Integrated Discovery (DAVID/EASE, http://david.abcc.ncifcrf.gov/). RNA-seq data have been deposited in the NCBI Gene Expression Omnibus database with accession number GSE55275. Separately, FPKM (fragments per kilobase of exon per million fragments mapped) values were computed using Cufflinks version 2.1.1 () and UCSC gene annotations. For additional information, see the supplementary Materials and Methods. […]

Pipeline specifications

Software tools DAVID, Cufflinks
Databases GEO
Application RNA-seq analysis
Organisms Homo sapiens