Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Dec 1 2013
Last update date: Mar 2 2014
Access: Public
Diseases: Brain Diseases
Genes: Ptbp2, PTBP2, PTBP1
Computational protocol: TopHat, Cufflinks, SpliceTrap, DAVID
Dataset link Microarray analysis of alternative splicing in PTBP2 knockout mouse brain

Experimental Protocol


Mice carrying a conditional floxed PTBP2 allele of PTBP2 were crossed to mice carrying Cre recombinase driven by the nestin promoter. The resulting knockout mutant mouse brains were analyzed for changes in gene expression and alternative splicing. Knockout mice were compared to wildtype littermates. Whole mouse brain polyA plus RNA was isolated from three Nestin-cre knockout embryos at embryonic day 18 and compared to three wildtype littermates. RNA was converted to cDNA and used to probe Affymetrix MJAY splicing sensitive microarrays and analysed by Omniviewer to identify changes in splicing.

Repositories


GEO

GSE51738

ArrayExpress

E-GEOD-51738

BioProject

PRJNA224738

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Areum Han

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