Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 10
Release date: Aug 13 2016
Last update date: Nov 26 2018
Access: Public
Genes: CIRBP, EBP50
Dataset link Temperature regulates splicing efficiency of the cold-inducible RNA-binding protein gene Cirbp

Experimental Protocol


Cultured NIH3T3 cells seeded and kept at 37C degree for 4 hours before being switched to 33C and 38C. After 16 hours of incubation the temperature was shifted to 38C and 33C, respectively. Sample were then taken at 0, 1, 3, 6 and 9 hour after the temperature shift. Paired-end, strand-specific, total RNA-seq was performed over the samples at the respective time points using the Illumina HiSeq2500 platform.

Repositories


GEO

GSE85553

ArrayExpress

E-GEOD-85553

ENA

SRP081516

BioProject

PRJNA338782

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Contact


Felix Naef