Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 43
Release date: Jan 11 2008
Last update date: Aug 28 2018
Access: Public
Diseases: Murine Acquired Immunodeficiency Syndrome
Dataset link Expression data from Murine T cell in response to IL-2 at 10 time points in 24 hours after IL-2 treatment

Experimental Protocol


Murine cytotoxic T lymphocyte cell line CTLL-2, derived from a C57BL/6 mouse, is an IL-2 dependent cell line. Cells were cultured in D10 medium in an incubator with 5% CO2 at 37oC to mid-log phase (~2 X 105 cells/mL), and collected by centrifugation, washed with phosphate buffered saline (PBS) (Invitrogen), resuspended at 1 X 106 cells/mL in the medium without IL-2 (IL-2 starvation) and cultured for 14 hours (No IL-2 stimulation, T0, 5 reps). After adding human rIL-2 (100 U/mL, Chiron, IL-2 stimulation), cells were collected at time point 0.5, 1, 2, 4, 6, 8, 10, 12, 16 and 24 hours, at least 3 biological replicates were carried out for each time point.Total RNA were extracted from the cells and was hybridized on Affymetrix GeneChip Mouse Genome 430 2.0 Array.

Repositories


GEO

GSE6085

ArrayExpress

E-GEOD-6085

BioProject

PRJNA97557

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Contact


Zhaoduo Zhang

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