Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 18
Release date: Dec 7 2017
Last update date: Nov 28 2018
Access: Public
Diseases: Urinary Bladder Neoplasms, Neoplasms
Chemicals: Tyrosine
Mutations: p|SUB|S|427|F
Dataset link Bladder-cancer-associated mutations in RXRA activate peroxisome proliferator-activated receptors to drive urothelial proliferation

Experimental Protocol


Two independent bladder cancer cell lines, JMSU-1 and 575A, were transfected with retrovirus to generate stable cell lines expressing either human wild-type RXRA (RXRAwt) or a mutant form of RXRA, RXRA S427F (RXRAS427F). RNA-seq was performed in biological triplicate on RXRAwt-expressing cells treated with vehicle or the PPARG agonist pioglitazone (pio), and RXRAS427F-expressing cells treated with vehicle. Fragments were sequenced on an Illumina HiSeq-2500 or HiSeq-3000. Transcriptomes in the wild-type and S427F conditions were compared to establish a causal role of the RXRA hot-spot mutation S427F in the hyper-activation of PPAR singling.

Repositories


GEO

GSE107735

ENA

SRP126244

BioProject

PRJNA421314

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Contact


Vivek Arora

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