Computational protocol: Cloning and characterization of filamentous temperature-sensitive protein Z from Xanthomonas oryzae pv. Oryzae

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Protocol publication

[…] The nucleotide and the predicted amino acid sequence were analyzed online (http://www.ncbi.nlm.nih.gov and http://www.expasy.org/proteomics), and the sequence comparison was conducted with the BLAST tool to find the homology of X. oryzae pv. Oryzae FtsZ with other FtsZ (http://www.ncbi.nlm.nih.gov). Swiss-Model analysis was also performed (http://swissmodel.expasy.org/). DNAMAN and MEGA5.1 software were used for sequence and phylogenetic analysis with the neighbour-joining method. […]

Pipeline specifications

Software tools SWISS-MODEL, DNAMAN, MEGA
Databases ExPASy
Applications Phylogenetics, Protein structure analysis
Organisms Xanthomonas oryzae, Escherichia coli BL21