Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 9
Release date: Oct 17 2008
Last update date: Aug 28 2018
Access: Public
Diseases: Leukemia, Mitochondrial Diseases
Dataset link Gene expression profile of mouse embryonic stem cells (D3-pOCT-mESC) grown in low concentrations of nitric oxide

Experimental Protocol


D3-pOct4 cells cultured in the presence of 1000 U/ml of LIF, in the absence of LIF, and in the absence of LIF supplemented with 2uM of diethylenetriamine nitric oxide adduct (DETA)/NO (Sigma) used as donor of nitric oxide. Treatments were maintained for seven days. RNA was extracted and analyzed by Bioanalyzer of Agilent. The cDNA microarray chip used was Mouse Genome 430_2.0 (Affymetrix) and the data were analyzed with GeneChip Operating System v1.4.0.036 using global scaling as the normalization method.

Repositories


GEO

GSE13235

ArrayExpress

E-GEOD-13235

BioProject

PRJNA109473

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Contact


Rafael Tapia Limonchi
Rafael A. Tapia Limonchi