Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 9
Release date: Oct 31 2010
Last update date: Jul 6 2016
Access: Public
Diseases: Heart Arrest
Dataset link Developmental Roles of MEC and NuRD Complexes in Caenorhabditis elegans.

Experimental Protocol


C. elegans L1 larvae treated with RNA interference were selected for RNA extraction and hybridization on Affymetrix microarrays. Synchronized wild type L4 animals were grown at 25° on bacteria expressing either gfp, let-418 or mep-1 dsRNA. Eggs were collected by bleaching gravid adults and allowed to hatch in the absence of food at 25°C. Newly hatched L1 larvae were fed on bacteria expressing the different dsRNA for three hours to recover from starvation. Three replicates per RNAi.

Repositories


GEO

GSE21376

ArrayExpress

E-GEOD-21376

BioProject

PRJNA126089

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Contact


Claude-Olivier Marti
Stefan Zoller