|Application:||Gene expression microarray analysis|
|Number of samples:||9|
|Release date:||Oct 31 2010|
|Last update date:||Jul 6 2016|
|Dataset link||Developmental Roles of MEC and NuRD Complexes in Caenorhabditis elegans.|
C. elegans L1 larvae treated with RNA interference were selected for RNA extraction and hybridization on Affymetrix microarrays. Synchronized wild type L4 animals were grown at 25° on bacteria expressing either gfp, let-418 or mep-1 dsRNA. Eggs were collected by bleaching gravid adults and allowed to hatch in the absence of food at 25°C. Newly hatched L1 larvae were fed on bacteria expressing the different dsRNA for three hours to recover from starvation. Three replicates per RNAi.