|Application:||RNA-seq analysis, miRNA array analysis|
|Number of samples:||12|
|Release date:||May 26 2016|
|Last update date:||Jul 26 2018|
|Dataset link||miRNA-mediated expression switch of cell adhesion genes driven by microcirculation in chip|
Undifferentiated Caco2 cells were seeded on individual polyether membrane inserts with 0.143 cm2 surface area and 1.0 μm pore diameter, cut out from HTS Transwell®-96 well permeable support (Corning Inc., USA), with cell density approximately 60,000 cells/well. The cells were incubated under conditions for differentiation for 7 days in MEM with 10% FBS, 0.1 mM non-essential aminoacids, 0.1% penicillin-streptomycin in 5% CO2, 37° C, changed three times. Transwell® inserts with CaCo2 monolayers were either kept on the microplate or put into microfluidic chip. The culture medium microcirculation parameters were ±20 kPa and 2 Hz resulting in the pulsatile (0 - 0.85 µl/s) medium flow with mean flow rate of 4.13 μl/min. After 24-h incubation the cells were lysed in 700 μl of Qiazol lysis reagent (Qiagen, Germany) and subjected to microarray expression analysis. The experiments were performed in triplicates.