|Number of samples:||103|
|Release date:||Dec 31 2015|
|Last update date:||Oct 24 2018|
|Dataset link||Biogenesis and function of tRNA fragments during sperm maturation and fertilization in mammals (ICSI)|
Zygotes were generated by ICSI from oocytes/females fed a Control diet and sperm/males fed either a Control or Low Protein diet. The sperm was isolated from either the Rete testis or the Cauda epididymis and injected either as a whole sperm or just the sperm head. Following fertilization by ICSI the zygotes developed for 28 hours (2C stage) and were harvested for single-embryo RNA-Seq.