Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 103
Release date: Dec 31 2015
Last update date: Oct 24 2018
Access: Public
Chemicals: Cholesterol
Dataset link Biogenesis and function of tRNA fragments during sperm maturation and fertilization in mammals (ICSI)

Experimental Protocol


Zygotes were generated by ICSI from oocytes/females fed a Control diet and sperm/males fed either a Control or Low Protein diet. The sperm was isolated from either the Rete testis or the Cauda epididymis and injected either as a whole sperm or just the sperm head. Following fertilization by ICSI the zygotes developed for 28 hours (2C stage) and were harvested for single-embryo RNA-Seq.

Repositories


GEO

GSE75769

ArrayExpress

E-GEOD-75769

ENA

SRP067085

BioProject

PRJNA305301

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Contact


Upasna Sharma