Dataset features


Application: Gene expression microarray analysis
Number of samples: 60
Release date: Oct 2 2010
Last update date: Aug 23 2018
Access: Public
Diseases: Neoplasms, X-Linked Combined Immunodeficiency Diseases
Chemicals: Benzo(a)pyrene
Dataset link In vitro carcinogenicity testing with Balb/c 3T3 Cells treated with various chemical carcinogens

Experimental Protocol

Balb/c 3T3 cells were seeded at 200 cells in each 60 x 15 mm culture dish with 4 ml M10F, using six culture dishes for every treatment. When cells reached a confluence of 60-65%, the culture medium was removed and replaced with fresh medium containing all tested chemicals at a specific concentration and two time points (24 and 120h). First we treated the cells both 24h and 120h with concentrations reported in the literature (0.5µM BaP, 50µM DAT, 25µM AAF and 2µM MCA). In a second approach IC20 concentrations were investigated for each chemical at both time points. The concentrations determined for IC20 ranged from 1.5 µM BaP to 700 µM DAT for 24h of treatment, and from 0.1 µM BaP or MCA to 10µM AAF for 120h of treatment. Balb/c 3T3 cells treated with 0.75% DMSO alone were kept as controls. Each experiment was run in triplicate.










Astrid Rohrbeck
Jürgen Borlak