Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Oct 6 2017
Last update date: Jan 24 2018
Access: Public
Dataset link Ectopic overexpression of the SnRK1 interacting protein STOREKEEPER RELATED 1/GeBP affects growth and development of Arabidopsis thaliana and leads to a constitutive immune response

Experimental Protocol


For microarray analyses, leaf samples from mature source leaves were taken in triplicate from 4-weeks old wild type plants or 35S-STKR1 lines 1, 2, or 5 either after 6 h of illumination or after an extended dark period for 6 h and total RNA was extracted using the RNAeasy Kit (Qiagen). Transcriptome profiling was performed on ArrayXS Arabidopsis v2 (XS-5010) microarrays in the Agilent 8 × 60K format (Oaklabs GmbH, Hennigsdorf, Germany) that represent 30,541 Arabidopsis genes. The array design is described in GEO accession GPL19779.

Repositories


GEO

GSE104652

BioProject

PRJNA413401

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Contact


Sophia Sonnewald