Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 6
Release date: Feb 23 2017
Last update date: Nov 26 2018
Access: Public
Diseases: Placental Insufficiency, Corneal Neovascularization
Chemicals: Oxygen
Dataset link Epithelial membrane protein 2 (EMP2) deficiency alters placental angiogenesis mimicking features of human intrauterine growth restriction.

Experimental Protocol


Day 13.5 placental tissue was isolated from Emp2+/+ and Emp2-/- animals and snap-frozen. Total RNA from each tissue was extracted using theRNAeasy kit (Qiagen. 3 samples per group were subjected to Illumina sequencing using the UCLA Pathology and Laboratory Medicine Core.  Libraries for RNA-seq were prepared with KAPA Stranded RNA-Seq Kit.

Repositories


GEO

GSE81940

ENA

SRP075761

BioProject

PRJNA323469

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Contact


Madhuri Wadehra