Dataset features


Application: Gene expression microarray analysis
Number of samples: 9
Release date: Aug 30 2012
Last update date: Mar 2 2015
Access: Public
Diseases: Glucose Metabolism Disorders
Chemicals: Cholesterol, Cytosine, Glucose
Dataset link Maternal slimming reprograms metabolic gene expression in mice offspring (NimbleGen expression)

Experimental Protocol

Female F0 founders were raised on a standard diet in a normal cage until 12 weeks of age, at which point they were placed into the enriched environmental cage or stayed in the normal cage (chosen at random) for 4 weeks. Males were always raised on a standard diet in the standard cage. At 16 weeks, female F0 founders were mated with males in standard conditions. After 1 or 2 days, males were removed, and pregnant females were left alone with a standard diet in the standard cage until their litters were 3 weeks of age. Note that we always used virgin males to avoid confounding effects brought about by the males. Moreover, males mated with two female groups did not differ in phenotypic data (body weight, adiposity, fasting blood glucose and insulin levels). At 3 weeks of age, partial offspring were sacrificed and the median lobe of liver was rapidly dissected out and flash-frozen in liquid N2, each from an independent mother. Samples from five control and four slimming offspring, each from different mothers, were chosen for microarray analysis.










yanchang wei