Dataset features

Specifications


Application: Gene expression microarray analysis, miRNA array analysis
Number of samples: 6
Release date: May 18 2013
Last update date: May 2 2017
Access: Public
Diseases: Neoplasms
Dataset link MicroRNA expression data from Bcl-xL silenced A549 lung adenocarcinoma cells

Experimental Protocol


Short interfering RNA-based transfection of A549 was carried out inducing a reduction in bcl-xL expression levels. 24 hours post-transfection total RNA was isolated using TRIzol reagent and hybridized onto Affymetrix GeneChip miRNA Arrays. A global miRNA expression profile was then established, which compared total RNA, extracted from siRNA-transfected and non-transfected A549 cells. All experiments were carried out with three independent biological replicates.

Repositories


GEO

GSE47059

ArrayExpress

E-GEOD-47059

BioProject

PRJNA203387

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Contact


Norahayu Othman

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