Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Apr 10 2014
Last update date: Jan 25 2018
Access: Public
Taxon: Danio rerio
Dataset link Perception of fight outcome is needed to activate socially driven changes in brain transcriptome

Experimental Protocol


Brain samples from zebrafish, Danio rerio were collected after experimental manipulations generated 4 phenotypes regarding social experience: mirror fighters (M), winners of a real opponent fight (W), losers of a real opponent fight (L); and socially isolated fish (I). After 30 minutes of experimental manipulation animals were anesthetized with a lethal dose of MS-222 (1000-1500 mg/l) and decapitated. Brains were rapidly collected, homogenized and RNA extracted following standard methods of RNeasy Lipid Tissue Mini Kit, Qiagen. RNA was then treated with DNase (RNase-free DNase set, Qiagen) to remove possible contaminations with genomic DNA and concentration and purity was estimated by spectrophotometric absorbance in a NanoDrop ND-1000 UV-Vis Spectrophotometer (Nano-Drop Technologies).

Repositories


GEO

GSE56549

ArrayExpress

E-GEOD-56549

BioProject

PRJNA243902

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