Computational protocol: The Intracellular Bacterium Wolbachia Uses Parasitoid Wasps as Phoretic Vectors for Efficient Horizontal Transmission

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Protocol publication

[…] Multi locus sequence typing (MLST) was used to identify Wolbachia in both AsiaII7 whitefly and E. sp. nr. furuhashii parasitoids. Five MLST genes, gatB (wMel locus WD_0146), coxA (WD_0301), hcpA (WD_0484), ftsZ and fbpA (WD_1238), together with the Wolbachia surface protein gene (wsp) were used for Wolbachia strain identification. The process is described in Baldo et al. [].Concatenated sequences of the seven most closely related Wolbachia sequence types (STs) associated with different host species were selected from the MLST database and used for comparisons with the STs isolated from other whitefly cryptic species, AsiaII7 and the parasitoid, E. sp. nr. furuhashii. Three STs from supergroup A, D and F Wolbachia were used as outgroups. The best model was chosen using the Bayesian Information Criterion (BIC) in MEGA5 []. Phylogenetic analysis based on the concatenated data of the five Wolbachia MLST loci (2079 bp) was undertaken using maximum parsimony (MP) and maximum likelihood in MEGA5 [] and PAUP version 4.0b []. T92+G model was used during ML tree constructions. […]

Pipeline specifications

Software tools MEGA, PAUP*
Application Phylogenetics
Organisms Bacteria, Wolbachia endosymbiont of Armadillidium vulgare str. wVulC, Bemisia tabaci
Diseases Infection