Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 9
Release date: Sep 17 2018
Last update date: Sep 19 2018
Access: Public
Chemicals: Iron
Dataset link Gene expression profiling of human HSCs treated with Eltrombopag

Experimental Protocol


Bone marrow was enriched for mononuclear cells by density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare, Pittsburgh, PA). Mononuclear cells were further enriched for CD34+ cells by immunomagnetic cell separation techniques (Miltenyi Biotec, Auburn, CA). Then hematopoietic stem cells (Lineage−CD34+CD38−CD49f+) were purified from CD34+ enriched cells by multiparameter-high speed fluorescence-activated cell sorting (FACS) on either FACS Aria II (Becton Dickinson, Franklin Lakes, NJ) or MoFlo Astrios (Beckman Coulter, Indianapolis, IN)

Repositories


GEO

GSE107428

BioProject

PRJNA420066

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Contact


Britta Will