Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 11
Release date: May 26 2011
Last update date: Oct 29 2018
Access: Public
Diseases: Neoplasms
Dataset link Antagonistic regulation of EMT by TIF1γ and Smad4 in mammary epithelial cells

Experimental Protocol


Total mRNA extractions were performed for 11 samples from transfected HMEC-TR. Replicates are rimo1, 6; rimo3, 9; rimo2, 7; rimo 4, 10 and rimo 5, 11. Rimo 8 is a single experiment. All RNA extractions were obtained from two independent cell cultures excepted for rimo8. Rimo 1, 6 are replicate for ctrl-; Rimo 3, 9 are replicate for ctrl+; Rimo 2, 7 are replicate for SiTIF-; Rimo 4, 10 are replicate for SiTIF+; Rimo8 is SiSmad4-; and Rimo5, 11 are replicate for SiSmad4+. ctrl means that HMEC-TR were transfected with an SiRNA scramble. "siSmad4" means that HMEC-TR were transfected with an SiRNA anti Smad4. "siTIF" means that HMEC-TR were transfected with an SiRNA anti TIF1γ. "-" means that cells were grown without TGFβ. "+" means that cells were treated with 5 ng/ml TGFβ1 for 24h.

Repositories


GEO

GSE28448

ArrayExpress

E-GEOD-28448

BioProject

PRJNA139143

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Contact


Cedric Hesling