Dataset features


Application: Gene expression microarray analysis
Number of samples: 36
Release date: Nov 3 2008
Last update date: Feb 5 2016
Access: Public
Diseases: Mastitis
Dataset link Gene expression profiling of liver from dairy cows subjected to intra-mammary LPS treatment: time course

Experimental Protocol

Eight healthy, high yielding Holstein-Friesian dairy cows in their first lactation (9 to 12 weeks after calving) were chosen for this study. At time 0 the right front quarter was infused with 200 μg E. coli LPS dissolved in 10 ml 0.9% NaCl solution, the left front quarter serving as control was infused with 10 ml 0.9% NaCl solution. Liver biopsies were taken at –22, 3, 6, 9, 12 and 48 hours relative to LPS infusion in 4 cows, and also at –22, 9 and 48 hours in the remaining 4 cows. RNA from liver biopsies was isolated and biotin labeled cRNA was loaded onto the Affymetric GeneChip Bovine Genome Array. A control study using cows infused with 0.9% NaCl showed that there was no effect of taking the biopsy, neither in the clinical measurement nor in the expression of a selected subset of genes. Therefore, only samples taken from the LPS treated cows were measured for the gene expression using microarrays.










Peter Sørensen
Peter Sørensen

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