Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Aug 29 2012
Last update date: Jul 6 2016
Access: Public
Dataset link Expression data in C. elegans L2 larvae after nhr-23 inhibition and in controls

Experimental Protocol


Synchronized populations of L1 larvae were plated with two sets of HT115 bacteria, one that had been transformed with the RNAi vector only (L4440 plasmid) and another that had been transformed with a vector targeting nhr-23 (clone 5174) [Proc Natl Acad Sci USA 98 (2001) 7360-7365]. Worms were kept on 2% agarose plates for 21 hr at 20C, collected, and approximately 200ml of worms resuspended in PBS were used in each individual experiment. Total RNA was isolated from frozen pellets using a Mixer-Mill (Miller-Mill 300) following an RNeasy Mini Kit (Qiagen, Germantown, MD) according to manufacturer protocol. Aliquots of cultures used for RNA isolation were kept on nhr-23 RNAi plates to confirm the knockdown phenotypic changes occurred during subsequent molts.

Repositories


GEO

GSE32031

ArrayExpress

E-GEOD-32031

BioProject

PRJNA147501

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Contact


WeiPing Chen