Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Oct 3 2016
Last update date: Mar 20 2018
Access: Public
Diseases: Infection
Chemicals: Polypropylenes, Silver
Dataset link A transcriptomic analysis of the bioactive properties of a polypropylene surgical mesh modified with silver-containing microparticles.

Experimental Protocol


New silver-containing permanent polypropylene meshes were compared with a conventional lightweight polypropylene mesh commonly used in abdominal wall reconstructive surgery). Meshes (3 sq cm each) were placed onto the pre-fascia avascular plane and fixed in 7-wk-old male Sprague-Dawley Hairless rats (n=8 per mesh). Half of the implanted meshes (n=4) were directly inoculated with a suspension of Staphylococcus aureus-Xen 29 strain obtained from the exponential phase of growth (107 CFU). After 10 days, rats were euthanized and the engrafted prostheses together with the surrounding tissues were explanted by cutting around the area. Half of each implant was frozen for total RNA extraction. Once isolated, biological pools each containing 3 individual replicates of an equal quantity of RNA from each animal were constructed. Gene expression was analyzed in 3 different pools per condition using the Affymetrix Rat Gene 2.1 ST 16-Array Plate.

Repositories


GEO

GSE85139

BioProject

PRJNA336317

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Contact


Maria Quiles