Similar protocols

Pipeline publication

[…] (). shows the clinicopathological features of the cancer patients according to the National Comprehensive Cancer Network (2012) guidelines (). Informed written consent was obtained from all patients and the study was approved by the Human Research Ethics Committee of The First Affiliated Hospital of Wenzhou Medical University., A total of 18 HCMV genomes were found and downloaded from the National Center for Biotechnology Information GenBank (http://www.ncbi.nlm.nih.gov/) and also from the University College London virus database (http://www.biochem.ucl.ac.uk/bsm/virus_database/VIDA_table_herpesviridae_cg.html). The similarity of each UL133–UL138 and IE1 coding sequence was examined using Basic Local Alignment Search Tool (http://blast.ncbi.nlm.nih.gov/Blast.cgi) and aligned by clustalx2 (ftp://ftp.ebi.ac.uk/pub/software/clustalw2/). The corresponding coding sequences in the varying strains were then used for homology analysis and specific primer design., Total RNA was extracted from frozen tissue specimens using TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s instructions. Next, the first-strand complementary DNA was reverse transcribed using 1 μg total RNA and the reverse transcription kit (Toyobo Co., Ltd., Osaka, Japan) according to the manufacturer’s instructions. The corresponding primers and the conditions of PCR amplification, as performed by the pre-programmed Thermal Cycler (Bio-Rad, Hercules, CA, USA), are listed in . Following amplification, the PCR products were subjected to electrophoresis in 2% agarose gel, stained with ethidium bromide, and images were captured u […]

Pipeline specifications

Software tools BLASTN, Clustal W, Primer Premier