|Application:||Gene expression microarray analysis|
|Number of samples:||23|
|Release date:||Nov 4 2016|
|Last update date:||Aug 23 2018|
|Dataset link||Functional roles of Acetylated Histone Marks at Mouse Meiotic Recombination Hotspots (expression)|
Wild-type meiotic samples design is described in GSE21447. To collect Spo11-/- meiotic samples, 10 to 16 weeks old male Spo11-/- testes were used for sorting multiple meiotic fractions. Spermatogonia, pre-Leptotene and Leptotene/Zygotene sorted meiotic fractions were collected twice from total 4 and 3 Spo11-/- mice testes respectively . These combined samples were used for subsequent RNA purification, labeling and hybridization to Affymetrix arrays.