Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 23
Release date: Nov 4 2016
Last update date: Aug 23 2018
Access: Public
Dataset link Functional roles of Acetylated Histone Marks at Mouse Meiotic Recombination Hotspots (expression)

Experimental Protocol


Wild-type meiotic samples design is described in GSE21447. To collect Spo11-/- meiotic samples, 10 to 16 weeks old male Spo11-/- testes were used for sorting multiple meiotic fractions. Spermatogonia, pre-Leptotene and Leptotene/Zygotene sorted meiotic fractions were collected twice from total 4 and 3 Spo11-/- mice testes respectively . These combined samples were used for subsequent RNA purification, labeling and hybridization to Affymetrix arrays.

Repositories


GEO

GSE57197

BioProject

PRJNA245895

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