Computational protocol: Common rs5918 (PlA1/A2) polymorphism in the ITGB3 gene and risk of coronary artery disease

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Protocol publication

[…] A common missense rs5918 (PlA1/A2) polymorphism in the ITGB3 gene was selected (http://www.ncbi.nlm.nih.gov/books/SNP). The four primers used in this research were designed by the online website Primer1: http://primer1.soton.ac.uk/primer1.html. The specificity of the primers was checked by the ‘BLAST’ program at http://www.ncbi.nlm.nih.gov/blast (). To enhance the specificity of inner primers, at the 3rd nucleotide from the 3’-terminus changes to destabilizing mismatch.SWISS-MODEL (http://swissmodel.expasy.org) was used for protein modeling of mutant protein. SWISS-MODEL is a fully automated protein structure homology-modeling server. The PyMol software was used for visualizing the effect of the altered residue on the protein structure. […]

Pipeline specifications

Software tools SWISS-MODEL, PyMOL
Databases ExPASy
Application Protein structure analysis
Diseases Coronary Artery Disease
Chemicals Nucleotides