Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 22
Release date: Apr 15 2015
Last update date: Oct 11 2016
Access: Public
Chemicals: Benzo(a)pyrene, Dexamethasone, Phenobarbital
Dataset link Integration of metabolic activation with a predictive toxicogenomics signature to classify genotoxic versus nongenotoxic chemicals in human TK6 cells [DEX]

Experimental Protocol


This experiment examined the whole genome transcriptional response of TK6 cells exposed to Dexamethasone for 4 hours followed by a 0h, 4h and 20h recovery in fresh media (4h, 8h, and 24h time points, respectively) at 2 different concentrations, including 0.63 mM and 1mM, in addition to negative controls (±S9), vehicle controls (±S9) and a direct-acting positive control, cisplatin (-S9) at 24 μg/ml (PC-24). Each concentration and time point had 3 biological replicates. There were a total 22 samples (44 arrays) included in the final analysis using a two-colour dye swap design. Please note that each sample data table contains normalized data combined from two replicates and the sample-dye assignment information for each of the raw data files is included in the 'readme.txt' (available as Series supplementary file).

Repositories


GEO

GSE51174

ArrayExpress

E-GEOD-51174

BioProject

PRJNA221483

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Contact


Julie Buick

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