Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Sep 1 2008
Last update date: Aug 28 2018
Access: Public
Genes: ATOH1
Dataset link Transcriptome analysis of the Arabidopsis phloem

Experimental Protocol


Arabidopsis plants were cultivated under short day conditions (8 h light) until flowering. Phloem tissue was isolated by Lasermicrodissection and Pressure Catapulting (LMPC) and phloem exudate by EDTA-chelation technique. In both experiments poly-A-RNA was extracted and amplified before hybridization of microarrays (Affymetrix, ATH1). For both experiments three LMPC-derived phloem tissue (LMPC-derived phloem_1-3) and three phloem exudate samples (Phloem exudate_1-3) were analysed. Precise protocols of plant growth, sample harvest, RNA extraction and amplification are provided in Deeken et al., 2008.

Repositories


GEO

GSE10247

ArrayExpress

E-GEOD-10247

BioProject

PRJNA108433

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Contact


Rosalia Deeken