Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Dec 5 2011
Last update date: Aug 23 2018
Access: Public
Diseases: Adenocarcinoma, Breast Neoplasms, Fibrocystic Breast Disease, Neoplasms
Chemicals: Doxycycline
Dataset link Transcriptional profiling of MMTV-tTA/TOP-ICN1 tumor derived cell lines comparing untreated (ICN1-On) cells to 24-hour doxcycline treated (ICN1-Off) samples

Experimental Protocol


Primary mammary tumors were isolated from two different MMTV-tTA/TOP-ICN1 transgenic mice, minced, enzymatically digested and converted to culture. To identify changes in gene expression in response to ICN1 suppression, tumor-derived cell lines 8534 and 8542 were left untreated (8534-Untreated; 8542-Untreated) or treated with 2ug/ml doxycycline for 24 hours (8534-Dox; 8542-Dox). Cells were collected by scraping and total RNA was isolated, followed by real-time PCR validation of NOTCH1 target gene modulation. RNA samples were further hybridized to Affymetrix mouse genome 430A2.0 arrays.

Repositories


GEO

GSE34146

ArrayExpress

E-GEOD-34146

BioProject

PRJNA150081

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