Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 9
Release date: Jun 15 2006
Last update date: Jan 18 2013
Access: Public
Diseases: Hyperglycemic Hyperosmolar Nonketotic Coma
Dataset link Role of Yap4 in Yeast Osmotic Stress Response

Experimental Protocol


Expt 1: The response of the wt to mild hyperosmolarity (0.4M NaCl) Serving as the exptal control, this experiment identifies the response of the wt strain to 0.4M NaCl. cDNAs obtained from total RNA extracted from wt cells subjected (test) or not (reference) to the indicated conditions for 10 minutes were labeled with Cy3 (reference) or Cy5 (test). Expt 2: Identification of potential Yap4 target genes under mild hyperosmolarity (0.4M NaCl). cDNAs obtained from total RNA extracted from wt (reference) and yap4 mutant (test) cells subjected to 0.4M NaCl for 10 minutes were labeled with Cy3 (reference) or Cy5 (test). Expt 3: The response of the yap4 mutant to mild hyperosmolarity (0.4M NaCl). cDNAs obtained from total RNA extracted from the yap4 mutant subjected (test) or not (reference) to 0.4M NaCl for 10 minutes were labeled with Cy3 (reference) or Cy5 (test).

Repositories


GEO

GSE5070

BioProject

PRJNA95507

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Contact


Claudina Rodrigues-Pousada