Dataset features


Application: miRNA array analysis
Number of samples: 8
Release date: Nov 13 2018
Last update date: Nov 13 2018
Access: Public
Diseases: Leukemia, T-Cell
Chemicals: Camptothecin
Taxon: Homo sapiens
Dataset link miRNA Profiles of Camptothecin-Sensitive and Resistant Jurkat T-Cells

Experimental Protocol

Jurkat T Lymphocytes were treated with 8 µM camptothecin for 4 hours to keep the population in early apoptotic phase, confirmed by immunoflorescent labeling. Treated and untreated populations were subjected to magnetic cell sorting. Four subfractions were obtained as untreated antiapoptotic (JNN-Jurkat Negative Negative), untreated apoptotic-which are naturally apoptotic- (JNP-Jurkat Negative Positive), camptothecin-treated apoptotic (JAP-Jurkat Apoptotic Positive) and camptothecin-treated antiapoptotic (JAN-Jurkat Apoptotic Negative). JNP fraction was not used for further analysis because of insufficient number of cells. Total RNA was extracted from all of the samples samples having higher RNA Quality due to RIN were chosen for miRNA array analysis. 2 replicates of JNN, 3 replicates of JAP and JAN were subjected to array analysis. 3 replicates of JNN fraction were mixed in 2 replicates because array analysis was performed in 8 channels.








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