Computational protocol: Taxonomy and physiological characterisation of Scheffersomyces titanus sp. nov., a new D xylose fermenting yeast species from China

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Protocol publication

[…] Comparisons with sequences from the international GenBank database (http://www.ncbi.nlm.nih.gov/) were conducted through BLASTN search. Sequences were aligned using the multiple sequence alignment program CLUSTAL X 1.83. Phylogenetic trees were constructed using MEGA software version 5.0. The evolutionary distance data were calculated from Kimura’s two-parameter model in neighbour-joining analyses. Confidence limits were estimated from bootstrap analysis (1000 replicates), and only values above 50% were recorded on the resulting trees. The sequences determined from this study, along with reference sequences obtained from GenBank are listed in . […]

Pipeline specifications

Software tools BLASTN, Clustal W, MEGA
Application Phylogenetics
Organisms Saccharomyces cerevisiae, Scheffersomyces stipitis
Chemicals Xylose