Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Feb 14 2013
Last update date: Oct 21 2013
Access: Public
Dataset link Transcriptomic response of the polycyclic aromatic hydrocarbon-degrading Sphingomonas sp. LH128 upon short-term starvation stress

Experimental Protocol


Transcriptomic response of phenanthrene degrading Sphingomonas sp. LH128 starved for 4h in isotonic solution of 0.01 mM MgS04 was studied using genome-wide gene expression analysis. For this purpose, the strain was pregrown in minimal medium to an OD600 of 0.5, washed twice with 0.01 mM MgS04 and resuspended in the same solution to an OD of 0.5. RNA was extracted both from starved cells and from the initial culture (non-starved cells) and cDNA was synthesized and labeled with Cy3. Transcriptomic response of three replicates were analyzed and compared with the initial inoculum

Repositories


GEO

GSE44310

ArrayExpress

E-GEOD-44310

BioProject

PRJNA189419

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