|Application:||Gene expression microarray analysis|
|Number of samples:||8|
|Release date:||Jan 26 2013|
|Last update date:||Aug 9 2018|
|Dataset link||Expression profiling of glioma initiating cells (GICs) in the sphere and differentiation conditions|
Within 4 hours of tumor removal from GBM patients, tissues were subjected to GIC preparation. After successive cloning, total RNA from GIC clones (GIC03A and GIC03U) on day 2 or 7 of subculture in NSC medium with or without 10% FCS was subjected to the analysis with Affymetrix microarrays. Simultaneously, the proteins extracted from the same set of cells were subjected to LC-shot gun analyses using the 8-plex iTRAQ method. We sought to obtain the information of the common molecules that were up- or down-regulated during the GSC differentiation process, and functional targets for the early onset of GIC-associated glioma.
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