Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Jul 1 2011
Last update date: Jul 11 2012
Access: Public
Genes: KRT83, AKR1C2
Taxon: Plasmodium falciparum
Dataset link Whole transcriptome analysis of rosetting Plasmodium falciparum parasites

Experimental Protocol


Plasmodium falciparum parasites, strain IT/R29, were selected for (R29R+) or against (R29R-) rosetting. Both cultures (R29R+ and R29R-) were tightly synchronised before a timecourse experiment was performed. 6 samples, named time points 1 to 6, were taken every 8 hours. 12μg of RNA from the R29 non-rosetting parasites at each of the 6 time points was combined together to form the reference pool. The pool and 12μg of each individual time point sample from both rosetting and non-rosetting parasites were then used for cDNA synthesis. For microarray hybridizations,each cDNA sample was coupled to Cy5 (red dye) while Cy3 (green dye) was added to the pool. Cy5-labelled time point samples were mixed with the same amount of Cy3-labelled pool sample. The solution was loaded on a microarray slide and hybridized for 14–16 h.

Repositories


GEO

GSE28990

ArrayExpress

E-GEOD-28990

BioProject

PRJNA140507

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Contact


Antoine Claessens

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