Computational protocol: Non-Cytotoxic Quantum Dot–Chitosan Nanogel Biosensing Probe for Potential Cancer Targeting Agent

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Protocol publication

[…] Fluorescence images were taken using Olympus IX71 epiluminescence microscope with a LUCPlanFLN 60×/0.70 ph2 objective lens (/0.1–1.3/FN22) (Olympus, Tokyo, Japan). Images were captured by Andor Zyla sCMOS (DG-152V-C1E-FI) camera (Andor, Oxford Instruments, Belfast, UK) with Micro-Manager 1.4 version software. Mercury lamp was used as an excitation source for FITC and Qdot fluorescence. For FITC images, excitation, dichroic mirror and emission filters (491/10 nm, 510 dclp, 514 ref, respectively) were used. For Qdot images, excitation dichroic mirror and emission filters were used respectively 360/40, 405lp, 585/20. The images were overlaid with ImageJ software (NIH, Bethesda, MD, USA). […]

Pipeline specifications

Software tools μManager, ImageJ
Application Microscopic phenotype analysis
Chemicals Cadmium, Cyanogen Bromide, Dopamine, Folic Acid, Glutathione, Fluorescein-5-isothiocyanate