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Three-dimensional macromolecule reconstruction software tools | Cryo-electron microscopy image analysis

Single-particle electron microscopy has now reached maturity, becoming a commonly used method in the examination of macromolecular structure. Using a small amount of purified protein, isolated molecules are observed under the electron microscope and the data collected can be averaged into a 3D reconstruction. Single-particle electron microscopy is an appropriate tool for the analysis of proteins that can only be obtained in modest quantities, like many of the large complexes currently of interest in biomedicine.

Source text:
(Llorca., 2005) Introduction to 3D reconstruction of macromolecules using single particle electron microscopy. Acta Pharmacol Sin.

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