Dataset features

Specifications


Application: 3C-seq analysis, Hi-C analysis
Number of samples: 23
Release date: Oct 30 2013
Last update date: Mar 29 2016
Access: Public
Dataset link High-resolution mapping of the spatial organization of Caulobacter crescentus chromosome by chromosome conformation capture in conjunction with next-generation sequencing (Hi-C)

Experimental Protocol


Hi-C experiments were performed on untreated wild type swarmer cells and drug-treated swarmer cells (Novobiocin or Rifampicin) of Caulobacter crescentus CB15N. Hi-C were also performed on swarmer cells of smc knock-out and hup1hup2 knock-out mutants of Caulobacter crescentus. Time-course Hi-C were performed on a synchronous population of Caulobacter crescentus at 5, 10, 30, 45, 60 and 75 minutes after synchronization. The progression of DNA replication during the cell cycle was followed by total genomic DNA sequencing.

Repositories


GEO

GSE45966

ArrayExpress

E-GEOD-45966

ENA

SRP020913

BioProject

PRJNA196826

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Contact


TUNG LE
TUNG BA KHANH LE