Dataset features

Specifications


Application: Gene expression microarray analysis, miRNA array analysis
Number of samples: 6
Release date: Oct 30 2009
Last update date: Mar 21 2012
Access: Public
Dataset link miRNA profiling from freshly harvested duodenums

Experimental Protocol


Duodenum samples were collected from mice, sliced into five identical pieces, transfered into eppendorf tubes and either processed immediately (T0) or maintained on ice and at latter time points [30 min (T30), 60 min (T60), 120 min (T120) and 240 min (T240)]. RNA was extracted by using Trizol and RNA integrity was assessed by Bioanalyzer electropherograms. We found that duodenal samples (which are rich in RNases) do show high susceptibility to degradation. These findings suggest that tissues such as duodenum or pancreas should either be processed immediately or snap-frozen and processed individually. miRNA expression profiling by microarray shows that miRNAs from freshly harvested duodenum are extensively degraded. Based on these data, we conclude that samples with low RIN values (less than 7) do not merit analysis on miRNA arrays.

Repositories


GEO

GSE18774

ArrayExpress

E-GEOD-18774

BioProject

PRJNA123831

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Contact


Mirco Castoldi

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