Computational protocol: Draft Genome Sequence of Bacillus murimartini LMG 21005T, an Alkalitolerant Bacterium Isolated from a Church Wall Mural in Germany

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[…] The type strain LMG 21005T of Bacillus murimartini was isolated from a church wall mural in Germany and identified as a novel alkalitolerant member of the Bacillus rRNA group 6 (). B. murimartini LMG 21005T showed 98.8% similarity with the closest described neighbor, Bacillus gibsonii DSM 8722T (). The optimal growth of B. murimartini LMG 21005T was achieved at 15°C to 20°C and pH 8.5; it was not salt tolerant (up to 4% NaCl) (). Notably, there is no other information about B. murimartini except its taxonomical description so far. Given no available genomic information of B. murimartini, its type strain LMG 21005T was selected as one of the research objects in our “genome sequencing project for genomic taxonomy and phylogenomics of Bacillus-like bacteria.” Here, we presented the high-quality draft genome sequence of B. murimartini LMG 21005T.The genome sequencing of B. murimartini LMG 21005T was performed via the Illumina HiSeq 2500 system. Two DNA libraries with insert sizes of 500 and 5,000 bp were constructed and sequenced. After filtering of the 1.33-Gb raw data, the 1.27-Gb clean data were obtained, providing approximately 200-fold coverage. The reads were assembled via the SOAPdenovo software version 1.05 (), using a key parameter K setting at 71. Through the data assembly, 13 scaffolds with total length 4,169,138 bp were obtained, and the scaffold N50 was 1,047,322 bp. The average length of the scaffolds was 320,702 bp, and the longest and shortest scaffolds were 1,178,572 and 508 bp, respectively. A total of 89.15% clean reads were aligned back to the genome, which covered 99.95% of the sequence,The annotation of the genome was performed using the NCBI Prokaryotic Genomes Automatic Annotation Pipeline (PGAAP) (http://www.ncbi.nlm.nih.gov/genomes/static/Pipeline.html) utilizing GeneMark, Glimmer, and tRNAscan-SE tools (). A total of 4,212 genes were predicted, including 4,120 coding sequences (CDS), 14 pseudogenes, 71 tRNAs, and 6 rRNA genes. There were 3,162 and 2,422 genes assigned to COG and KEGG databases, respectively. The average DNA G+C content was 43.40%, with a significant difference from the value 39.6 mol% acquired by or high-performance liquid chromatography (HPLC) determination (). […]

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