Computational protocol: Tissue-derived mesenchymal stromal cells used as vehicles for anti-tumor therapy exert different in vivo effects on migration capacity and tumor growth

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Protocol publication

[…] Animals bearing the tumor xenograft were separated into six groups (n = 4/group) when the tumors reached 50 mm3. MSCs were labeled with SPIO as described above. Groups 1 to 5 received an intravenous injection of 106 SPIO-labeled MSCs, while the control group (group 6) received intravenous injection of PBS. Scans were performed at 3, 10, 17, and 24 days after injection. The MRI experiments were performed (Pharmascan system; Bruker Medical GmBH, Germany, using a 7.0-T horizontal-bore superconducting magnet, equipped with a 1H selective surface coil and a Bruker gradient insert with 90 mm inner diameter (maximum intensity 360 mT/m). All data were acquired using Paravision software (Bruker). Anesthesia was initiated using oxygen (1 l/min) containing 4% isofluorane, and maintained during the experiment with 1 to 1.5% isofluorane in O2. T2-weighted spin-echo anatomical images were acquired by rapid acquisition with relaxation enhancement (RARE) sequence in axial (12 slices) and sagittal (8 slices) orientations and the following parameters: TR 3000 ms, TE 60 ms, RARE factor 8, average 3, FOV 30 × 30 mm, acquisition matrix 256 × 256, corresponding to an in-plane resolution of 117 × 117 μm2 and slice thickness of 1.00 mm. Tumors were measured every 2 days and tumor volume was calculated using the formula:tumor volume = 1/2 L × S2, where L is long side and S is short side. [...] Results are reported as mean ± SEM. Statistical evaluation of data was carried out using the SPSS Statistics software package (version 17.0; IBM SPSS, Chicago, IL, USA). Normal distribution of the variables was analyzed by means of the Kolmogorov-Smirnov test followed by the Tukey HSD test, except for KLF4 and REX1 expression in the pluripotency qPCR assays, whose distribution was non-parametric and so analysis was performed using the Kruskal-Wallis test. P<0.05 was considered significant. […]

Pipeline specifications

Software tools ParaVision, SPSS
Applications Miscellaneous, Magnetic resonance imaging
Organisms Homo sapiens
Diseases Neoplasms