|Number of samples:||12|
|Release date:||Sep 30 2016|
|Last update date:||Jun 7 2017|
|Dataset link||Single-peptide DNA-dependent RNA polymerase homologous to multi-subunit RNA polymerase|
The wildtype and ΔyonO strains were grown in MMB media until exponential phase, at which point the cultures were halved. To one half (the plus sample), mitomycin C was added to induce the SPβ prophage. The other half (the minus sample) was left uninduced. Both cultures were incubated for a further 60 minutes before cells were harvested. Total RNA was purified from harvested cells and used for RNAseq analysis. To provide biological replicates, each strain was grown, induced and harvested in triplicate.