Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 12
Release date: Sep 30 2016
Last update date: Jun 7 2017
Access: Public
Chemicals: Amino Acids
Dataset link Single-peptide DNA-dependent RNA polymerase homologous to multi-subunit RNA polymerase

Experimental Protocol


The wildtype and ΔyonO strains were grown in MMB media until exponential phase, at which point the cultures were halved. To one half (the plus sample), mitomycin C was added to induce the SPβ prophage. The other half (the minus sample) was left uninduced. Both cultures were incubated for a further 60 minutes before cells were harvested. Total RNA was purified from harvested cells and used for RNAseq analysis. To provide biological replicates, each strain was grown, induced and harvested in triplicate.

Repositories


GEO

GSE80786

ENA

SRP074147

BioProject

PRJNA319983

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Contact


Simon Cockell

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