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Specifications


Dataset type: Other
Number of samples: 12
Release date: Feb 28 2018
Last update date: Nov 29 2018
Access: Public
Chemicals: N-Acetylneuraminic Acid
Dataset link Genome-Wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication

Experimental Protocol


A pooled library of A549 cells lacking individual genes was generated using CRISPR/Cas9 gene deletion technique (A549-GeCKO library; genome-wide CRISPR knockout library). The single guide RNA (sgRNA) representation in the A549-GeCKO library was sequenced. The A549-GeCKO library was subjected to 5 consecutive rounds of infection with a human isolate of low pathogenic H5N1 influenza virus and the sgRNA enriched in the resistant population were sequenced (Prelim Rd 5). In addition, a sequential screen was performed in biological duplicates and the sgRNA eriched after each round of infection were sequenced (Rd 1-5, Rep1-2 )

Repositories


GEO

GSE111166

ENA

SRP133553

BioProject

PRJNA436088

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Contact


Yan Li

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