Dataset features


Application: Gene expression microarray analysis
Number of samples: 11
Release date: Mar 9 2009
Last update date: May 21 2015
Access: Public
Chemicals: Nucleotides
Dataset link Characterization of the Ku70 homologue HdfA deletion in Penicillium chrysogenum

Experimental Protocol

Penicillium chrysogenum is a filamentous fungus that is used to industrially produce the beta-lactam antibiotics. After decades of classical strain improvement, the genome sequence has been deciphered and annotated. The combination of the P. chrysogenum genome sequence and a strain exhibiting increased targeting efficiency would paved the way to the construction of deletion or expression libraries that would be a tremendous help in understanding the nature of the productive capabilities of this organism. This study reports the effect of the deletions of the Ku70 and Ku80 homologues, HdfA and HdfB respectively, in P. chrysogenum. A detailed analysis of the physiology, transcriptome and phenotype was performed to assess the use of these strains as platform for functional genomics study.








Jean-Marc Daran