Dataset features


Application: Gene expression microarray analysis
Number of samples: 14
Release date: Jun 26 2007
Last update date: Mar 17 2012
Access: Public
Diseases: Neoplasms, Scleroderma, Limited
Dataset link Low-level MMS treatment of S.cerevisiae

Experimental Protocol

YPD and F1 media were inoculated with overnight pre-cultures and grown at 30°C to mid log-phase (OD600 0.6 to 0.8). Cultures were split into three parts: the first aliquot was mock-treated and used as control; the second and third aliquots were treated with low concentration of MMS (0.00125% and 0.0125%). All cultures were incubated at 30° C. Samples were collected after 30 min and 1h incubation. The total of 14 samples were analysed. With the FF18984 strain experiments were done in minimal (F1) and full (YPD) medium with both MMS concentrations (0.00125% and 0.0125%) for 30 min and 1h. With the BY4742 strain experiments were done in full (YPD) medium with 0.0125% MMS for 1h. As a reference we used mock-treated samples from the same medium and the same time point.








Ana Kitanovic