Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 8
Release date: Aug 28 2013
Last update date: Oct 24 2018
Access: Public
Genes: Pou5f1-rs1, LuxS, Per2, NCUBE1, IL6, POU5F1, KLF4, SOX2, MYC
Taxon: Mus musculus
Dataset link Global transcriptome profiling of Oct4/Klf4/Sox2 (3Factor, 3F) + IL6 iPS clones derived from mouse embryonic fibroblasts.

Experimental Protocol


This study includes 8 samples: 2 independently derived 3F + IL6 iPS clones, 2 independently derived 4F iPS clones, 2 biological replicates of mouse D3-GFP ES cells, and 2 biological replicates of mouse embryonic fibroblasts (MEFs). The latter 6 samples are provided as references for the 3F + IL6 iPS clones. Poly-A RNA was isolated and prepared for sequencing using the Illumina TruSeq RNA kit (v2) to generate 50bp reads. Reads were aligned to mm10.

Repositories


GEO

GSE46104

ArrayExpress

E-GEOD-46104

ENA

SRP021101

BioProject

PRJNA197269

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