Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 63
Release date: Oct 5 2015
Last update date: Oct 17 2018
Access: Public
Diseases: Deficiency Diseases
Dataset link A mechanism for expansion of the regulatory T cell repertoire and its role in enforcing self-tolerance

Experimental Protocol


DO11.10 TCRβ transgene inhibits the recombination of endogenous Tcrb loci thus restricting TCR repertoire to TCRα chains expressed by T cells. Further limitation of the TCR repertoire was achieved by the presence of one functional Tcra gene. With restricted TCR repertoire, mRNA of TCRα was extracted from Treg and conventional CD4+ T cells for library preparation and high throughput sequencing.

Repositories


GEO

GSE71162

ENA

SRP061377

BioProject

PRJNA290476

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Contact


Alexander Rudensky

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