|Number of samples:||63|
|Release date:||Oct 5 2015|
|Last update date:||Oct 17 2018|
|Dataset link||A mechanism for expansion of the regulatory T cell repertoire and its role in enforcing self-tolerance|
DO11.10 TCRβ transgene inhibits the recombination of endogenous Tcrb loci thus restricting TCR repertoire to TCRα chains expressed by T cells. Further limitation of the TCR repertoire was achieved by the presence of one functional Tcra gene. With restricted TCR repertoire, mRNA of TCRα was extracted from Treg and conventional CD4+ T cells for library preparation and high throughput sequencing.
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