Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 21
Release date: Jul 30 2009
Last update date: Jul 8 2016
Access: Public
Diseases: Latex Hypersensitivity
Dataset link Gene expression profiling of patients with allergy to latex and/or vegetable food

Experimental Protocol


This study aimed at the understanding of the molecular pathways involved in allergy to latex and fruit. As latex and vegetable food allergens cross-react, it could be hypothesized that similar pathways are involved in both types of hypersensitivity. For this purpose, patients allergic to latex (n=6), allergic to vegetable food (n=5), or suffering from latex-fruit syndrome (n=6) were enrolled from the Unit of Allergy of the Gemelli Hospital of Rome. Moreover, 4 healthy volunteers were added to this study. Five ml of blood were harvested from each individual, and PBMCs were isolated on ficoll gradient. Following sample preparation (as recommended by the manufacturer), each sample was hybridized on Affymetrix human focus array. Data were processed with Affymetrix MAS 5.0 software and averaged intensity of signals from biological replicates were calculated for further analysis. The CEL files for this study were lost in a computer crash.

Repositories


GEO

GSE13619

ArrayExpress

E-GEOD-13619

BioProject

PRJNA110643

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Contact


Nathalie Saulnier